T-DNA of a crown gall tumor is organized in nucleosomes

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DNA sequence analysis of crown gall tumor T-DNA encoding the 0.7 kb transcript.

Crown gall tumor formation involves integration into the plant genome of DNA sequences (the T-region) of tumor-inducing (Ti) plasmids present in Agrobacterium tumefaciens. The T-DNA of the tumor expresses several gene products. Little is known about the function or regulation of expression of the 0.7kb transcript, which represents a relatively abundant T-DNA transcript in octopine-type tumors. ...

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Unusual plasmid DNA organization in an octopine crown gall tumor.

A cloned tobacco crown gall tumor, 1595501, incited by A. tumefaciens strain 15955 was studied. Molecular analysis of the organization of the T-DNA by means of Southern transfer and hybridization techniques indicated that the 1595501 tumor has about 10 copies of TL DNA, five of which are complete TL DNA, whereas most octopine tumors have only one to two copies of complete TL DNA. Hybridization ...

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Recovery of crown-gall tumor cells.

nant animal cells are distinguished from healthy or inflaznmstory cells. In emphasizing the perma nency of the cellular alteration, Kidd (6) states that “Cancercells remain such until they die . . they never turn back en masse towards the benign or normal state. . . .“The inability to accomplish a reversal of malignant to normal cells under con trolled conditions has, however, served as a s...

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Hormonal control of tobacco crown gall tumor morphology.

The endogenous levels of auxin and cytokinin in teratoma and unorganized tobacco (Nicotiana tabacum L. var Wisconsin #38) crown gall tumor tissues were determined. Teratoma tissues contain levels of auxin and cytokinin favorable for shoot formation, whereas unorganized tumors contain levels of auxin that suppress shoot formation. This conclusion is based upon the observation that when levels of...

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DNA Methylation Mediated Control of Gene Expression Is Critical for Development of Crown Gall Tumors

Crown gall tumors develop after integration of the T-DNA of virulent Agrobacterium tumefaciens strains into the plant genome. Expression of the T-DNA-encoded oncogenes triggers proliferation and differentiation of transformed plant cells. Crown gall development is known to be accompanied by global changes in transcription, metabolite levels, and physiological processes. High levels of abscisic ...

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ژورنال

عنوان ژورنال: The EMBO Journal

سال: 1984

ISSN: 0261-4189

DOI: 10.1002/j.1460-2075.1984.tb01814.x